Engineered Serum and Alternatives


Promote growth with the cost-effective HyClone Alpha Calf Fraction media supplement, designed for antibody production in hybridoma cell cultures.

  • Cost-effective medium supplement optimized for hybridoma cells
  • Sourced in the USA with complete traceability back to the original source.
  • Low in antibodies and high in growth factors
  • Virus panel testing according to 9 CFR 113.53

Alpha Calf Fraction is filtered through three sequential 100 nm (0.1 µm) pore size-rated filters. Before dispensing, each lot of serum is pooled using true pool technology to ensure uniformity and consistency between bottles. Produced from bovine calf serum, using a non-ethanol proprietary process to reduce immunoglobulins and protein to levels very similar to those of Fetal Bovine Serum (FBS). Alpha Calf Fraction typically contains less than 200 μg IgG /mL.

Additional treatments available including heat-inactivation and irradiation. Additional testing, including EMEA-conforming testing, is also available.

PRODUCT CODE PRODUCT NAME PACKSIZE
SH30076.03 Alpha Calf Fraction Supplement (U.S.) Iron-Supplemented 1
SH30212.03 Alpha Calf Fraction Supplement (U.S.) 1

HyClone Bovine Growth Serum (BGS) was developed as an affordable and viable alternative to Fetal Bovine Serum (FBS).

  • Sourced in the USA with complete traceability back to original source
  • Cost-effective alternative to FBS
  • Growth promotion studies demonstrate that BGS is an excellent replacement for FBS
  • Low in antibodies and high in growth factors
  • Virus panel testing according to 9 CFR 113.53

BGS consists of high-quality bovine calf serum supplemented with chemically defined components including vitamins, amino acids, trace metals, and other small molecules to stimulate cell growth and proliferation.

Bovine Growth Serum is filtered through sequential 100 nm (0.1 µm) pore size-rated filters. Before dispensing, each lot of serum is pooled using true pool technology to ensure uniformity and consistency between bottles.

Additional treatments available including heat-inactivation and irradiation. Additional testing, including EMEA-conforming testing, is also available.

PRODUCT CODE PRODUCT NAME PACK SIZE STORAGE
SH30541.02 Bovine Growth Serum (U.S.) 100 mL Sera should be stored at -10°C or lower. Once thawed, sera should be stored at 2° to 8°C.
SH30541.02HI Bovine Growth Serum (U.S.) HEat Inactivated 100 mL
SH30541.03 Bovine Growth Serum (U.S.) 500 mL
SH30541.03HI Bovine Growth Serum (U.S.) HEat Inactivated 500 mL
SH30541.03IR Bovine Growth Serum (U.S.) Irradiated 500 mL

Optimized for the growth of hybridoma cells.

  • Optimized for the growth of hybridoma cells
  • IgG levels comparable with those found in FBS
  • Demonstrated cell growth performance equivalency to FBS
  • High lot-to-lot consistency
  • Low endotoxin and hemoglobin levels

Sterile filtered using three sequential 100 nm (0.1 µm) pore-size rated filters. Each lot undergoes extensive quality performance and analytical testing

Additional treatments available including heat-inactivation and irradiation. Additional testing, including EMEA-conforming testing, is also available.

PRODUCT CODE PRODUCT NAME PACK SIZE
SH30080.02 FetalClone I Serum (U.S.) 100 mL
SH30080.02HI FetalClone I Serum (U.S.) Heat Inactivated 100 mL
SH30080.03 FetalClone I Serum (U.S.) 500 mL
SH30080.03HI FetalClone I Serum (U.S.) Heat Inactivated 500 mL
SH30080.03IR FetalClone I Serum (U.S.) Irradiated 500 mL

Optimized for the growth of CHO cells and derivatives.

  • Optimized for the growth of CHO cells and derivatives
  • Offering the same basic formulation as our FetalClone I, with additional growth factors and supplements
  • IgG levels comparable with those found in FBS
  • Improved cell growth performance relative to FBS
  • High lot-to-lot consistency
  • Low endotoxin and hemoglobin levels

U.S. Sourced HyClone FetalClone II engineered serum is an economical alternative to Fetal Bovine Serum (FBS), delivering both cost-effectiveness and high performance.

Sterile filtered using three sequential 100 nm (0.1 µm) pore-size rated filters. Each lot undergoes extensive quality performance and analytical testing

Additional treatments available including heat-inactivation and irradiation. Additional testing, including EMEA-conforming testing, is also available.

PRODUCT CODE PRODUCT NAME IGG LEVELS PACK SIZE
SH30066.02 FetalClone II Serum (U.S.) Typically less than 2 ug/mL 100 mL
SH30066.02HI FetalClone II Serum (U.S.) Heat Inactivated Typically less than 2 ug/mL 100 mL
SH30066.03 FetalClone II Serum (U.S.) Typically less than 2 ug/mL 500 mL
SH30066.03HI FetalClone II Serum (U.S.) Heat Inactivated Typically less than 2 ug/mL 500 mL
SH30066.03IR FetalClone II Serum (U.S.) Irradiated Typically less than 2 ug/mL 500 mL

An economical alternative to Fetal Bovine Serum (FBS).

  • The most widely applicable of our FetalClone family of products, designed to include fibroblasts in its applications
  • Offering the same basic formulation found in FetalClone II, with additional growth factors and supplements
  • IgG levels comparable with those found in FBS
  • Improved cell growth performance relative to FBS
  • High lot-to-lot consistency
  • Low endotoxin and hemoglobin levels

U.S. Sourced HyClone FetalClone III offers the same basic formulation of FetalClone II with additional growth factors and supplements.

Sterile filtered using three sequential 100 nm (0.1 µm) pore-size rated filters. Each lot undergoes extensive quality performance and analytical testing

Additional treatments available including heat-inactivation and irradiation. Additional testing, including EMEA-conforming testing, is also available.

PRODUCT CODE PRODUCT NAME PACK SIZE
SH30109.02 FetalClone III Serum (U.S.) 100 mL
SH30109.02HI FetalClone III Serum (U.S.) Heat Inactivated 100 mL
SH30109.03 FetalClone III Serum (U.S.) 500 mL
SH30109.03HI FetalClone III Serum (U.S.) Heat Inactivated 500 mL
SH30109.03IR FetalClone III Serum (U.S.) Irradiated 500 mL